The experimental studies were conducted in two leading African malaria vector control institutions: Ifakara Health Institute (IHI), Tanzania and Institut de Recherche en Sciences de la Santé (IRSS), Burkina Faso. In IHI, the study experiments were conducted in the mosquito biology laboratory Vector Sphere and the larvae were collected from three villages in the Kilombero floodplains in Ulanga district, south-eastern Tanzania: Minepa village (8.285S, 36.669E), Tulizamoyo village (8.348S, 36.732E), and Sululu village (8.003S, 36.832E). The ecology and species available in Ulanga district were recently described29. In IRSS, mosquito sampling was conducted in the north of Bobo-Dioulasso in Vallée du Kou village VK5 village (4.4201W, 11.3824N) and in the south of Bobo-Dioulasso in Soumousso (4.0438W, 11.0125N).
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